Journal: Journal of Nanobiotechnology

Article Title: Hydrogen-generating nanomodulators for metabolic repair and functional recovery in ovarian tissue transplantation

doi: 10.1186/s12951-026-04024-x

Figure Lengend Snippet: KBH₄@Gel improves early follicle survival and promotes angiogenesis in transplanted ovarian tissue. ( A ) Experimental Procedure and Grouping. ( B ) Representative H&E and TUNEL staining of ovarian grafts on Day 7 post-transplantation. ( C ) VEGF immunohistochemistry. ( D ) CD34 immunohistochemistry. ( E ) Quantitative analysis of primordial follicle counts, follicle apoptosis rate, VEGF-positive area, and microvessel density. The scale bar is 50 μm. Data are presented as mean ± SD and analyzed using an unpaired two-tailed Student’s t-test (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; ns, not significant)

Article Snippet: Sections were incubated with primary antibodies against CD34 (1:200, Proteintech, Wuhan, China) or VEGF (1:2500, Proteintech, Wuhan, China), followed by EnVision HRP-conjugated secondary antibody and visualization with liquid DAB substrate (Dako, Carpinteria, CA, USA).

Techniques: TUNEL Assay, Staining, Transplantation Assay, Immunohistochemistry, Two Tailed Test

Journal: Asian Journal of Pharmaceutical Sciences

Article Title: Lignin-assisted construction of sub-10 nm supramolecular self-assembly for photothermal immunotherapy and potentiating anti-PD-1 therapy against primary and distant breast tumors

doi: 10.1016/j.ajps.2022.07.002

Figure Lengend Snippet: In vivo immune responses assessment. (A) Flow cytometry assay of CD80 + or CD86 + gated on CD11c + in the TDLNs of mice. (B) Flow cytometry assay illustrated the percent of CD8a + T cells and CD4 + T cells gated on CD3 + T cells in splenocytes of mice. (C-D) Quantitative percentages of CD80 + or CD86 + expressions gated on CD11c + according to (A). (E) Quantitative percentages of CD8a + T cells and CD4 + T cells in splenocytes according to (B). (F-H) Quantitative analysis of the immune-associated cytokines in serum, as determined by ELISA (IL-2, TNF-α and IFN-γ). ( n = 3, mean ± SD, * P < 0.05, ** P < 0.01 and ## P < 0.0001).

Article Snippet: Fluorochrome-labeled anti-mouse monoclonal antibodies (CD11c-allophycocyanin (APC), CD80-fluorescein isothiocyanate (FITC), CD86-phycoerythrin (PE), CD3-FITC, CD8a-PE, and CD4-APC) were bought from Proteintech.

Techniques: In Vivo, Flow Cytometry, Enzyme-linked Immunosorbent Assay

Journal: Bioactive Materials

Article Title: Novel magnetic silk fibroin scaffolds with delayed degradation for potential long-distance vascular repair

doi: 10.1016/j.bioactmat.2021.04.036

Figure Lengend Snippet: Immunohistochemistry stained (A) and statistics (B) in different scaffolds (SFC, MSFC10 and MSFC250) with CD34 antibody after 1 W, 2 W, 4 W and 16 W implantation, n = 3; (C) In vivo degradation of different scaffolds (SFC, MSFC10 and MSFC250), representative pictures corresponding to each time point were presented. *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA test.

Article Snippet: The obtained tissue sections (10 μm sheet thickness) were co-incubated with primary CD34 (1: 200) antibody (Rabbit Polyclonal, proteintech, China) overnight at a 4 °C refrigerator.

Techniques: Immunohistochemistry, Staining, In Vivo